Transcribed
So we've all been at this point before we fruit on mushrooms. We go to harvest. And one of them mushrooms is just absolutely picture perfect. But the other mushrooms don't look like that. And you're thinking to yourself, man, I wish there was a way to make every single mushroom knife fruit look exactly like this one. Eliminate the runts and only keep the big boys. Well, matter of fact, there is a way to do that. And I'm going to show you how to do that right now.
Trevino, as they know, notes arriving every day will be held off as regear Bobby Loden in Athas Mason 32s. You can really add a future somebody.
Man What's going on? Trip team first of all, I want to welcome you guys back to a brand new tech video. I know a lot of you guys want these tech videos and I'm going to give them to you guys. It's going to be a mix of tax blog, psychedelic journeys, spirituality, all that good shit. I want to give you guys a well-rounded buffet of psychedelic knowledge. That's what Willie mikos here to do and that's what my track team family wants. So if you like this video and you love Willie micro- and you're a real trip team family supporter, smash that like comment down below and definitely hit that subscribe button. We want to get them numbers up. I love you guys. You love me. So show you love by and all them buttons. Takes two seconds. Do it right now. Also, make sure you guys check me out on all my social media. Tons of content behind the scenes, extra stuff, giveaways, all that good stuff. So make sure you go check it out. Now that we've got all that corny YouTubers, self-promotion, garbage out of the way. Let's jump into this video. So today we're gonna be talking about cloning. I'm going to show you guys my preferred methods. I'm going to show you two different methods that you guys could use. We're also going to talk about the pros, the cons, why people do it, what you can do it with and all that good stuff. We're gonna get into all the details, just like I like to give it to you guys. So make sure you guys sit back, enjoy and follow along. So with that said, let's jump right into this, because we have a lot to cover. Let's talk about what we are going to need to follow along with this video.
All right. Chip team. So you're about to start your cloning process and you need a selected donor fruit. That's the first thing you want to do. Now, some things might seem really obvious and there's others that really ain't in. I've learnt a lot of different things over the years that have helped me get great results. And I'm going to share that with you guys. Now, most people, when they go to select a fruit, they're looking for the biggest and baddest mofo out of the entire flesh. And that's pretty simple and straightforward. But another thing you want to pay attention to is once you select that big mushroom that you want to start cloning, what is the immediate surrounding fruits look like? Are they consistent? Do they look good? Is there a lot of aborts? You know, you want to pay attention to this. You want to try to find a fruit that's surrounded by other nice looking consistent fruits because they share a lot of the same genetics. So it's very important that you pay attention to not only the fruit itself, but the surrounding fruits. Another couple of things that people want to look for is mutations. Is there a different colored spores? Is it in our Bible? Was it a rust spore, you know? Is there something special about the way the cap connects to the veil or the shape of the cap? Is there anything that sets the fruit apart? You know, when you're looking for that perfect fruit, it might not always be the biggest, baddest fruit.
It might have something very special about it, like its coloration or the spore coloration or there's something specific and unique about the spores themself. So you want to keep that in mind as well, because that's going to play a big part in your cloning process. Now, once you guys have selected that perfect fruit, you know, that fruit that you want to start cloning, you guys are all set and you can move forward. The next thing you guys are going to need is you're a garden dishes. Now, there's different types of agar, but I actually have videos out showing you how to make LME a yard dishes if you guys don't want to make them yourself. You could purchase them pre port, but they're very easy, very simple to make yourself and you're going to need a lot of them as you do transfers. So it's very good if you guys know how to make them yourself. You'll save a lot of money and a lot of time. The next thing you guys are going to need is a lighter and or an alcohol burning lamp. Now, alcohol burning lamps are relatively cheap. You guys could purchase them on Amazon from shrimp supply dot com. You guys can just use a light and a flame sterilized. I'm sure you guys, if you were watching this video, you already know how to flame sterilize.
And next item you guys are going to need is a scalpel with blades. Now, there's different types of scalpels and blades out there, but I have my scalpel and then I buy disposable blades. So they come in single sterile packs just like this. You guys could either use that or you guys could buy a scalpel that already has the blade attached to it and just use. Now, the next thing you guys are going to need is some isopropyl alcohol comes in 70 percent, 80 percent, 90 percent. I tried to go for the highest percentage I could find. You guys are also going to need some vinyl or latex gloves and some hand sanitizer. You guys are going to need a few sheets of paper towels, have a few on hand ready to go. And of course, you guys are going to need the fruit that you selected in the beginning. Now, I selected a couple of fruits because I'm going to show you two different methods of cloning. But there's really nothing special about these fruits. These are just pretty much basic fruits that I'm just using as an example for this video. If you guys out there wanting to clone something of your own, trying to make sure it's something special you guys don't want to do is clone any basic old fruit.
Now, obviously, before you guys start any process, whether it's laying here, a guard dish is taking a tissue sample, making a sports arrange anything and mycology. You guys want to be working inside of a still air box or underneath some type of filtration system like I am. I also like to take a shower and scrub really good before I start any process. This is going to help reduce the risk of contamination or, you know, any foreign debris or bacteria. Get in on your agar dishes. So just think every step of the way. Sterile, sterile, sterile. It's gonna help you guys out a lot. And that's an a really reduce your risk of failure. Now, the first thing you guys want to do is you want to put your latex or your vinyl gloves on. Try to use gloves that a powder free.
Once you guys have your gloves on, put your hand sanitizer on and sanitize your gloves extremely well. Next, do you want to remove your scalpel blade from the packaging? And you want to put it on your scalpel. Be very careful not to cut yourself. These could get slippery, especially with the gloves on. Once you guys have your scalpel blade on the scalpel, then you want to take some rubbing alcohol, put it on one of your paper towels or cotton swab and you want to start to wipe down your scalpel. Even though it just came out of a sterile pack, you still want to wipe down the blade as well. Next, take the alcohol burning lamp or your lighter. Then what you wanna do is want to flame sterilize your scalpel blade. You want to flame, sterilize it into little red hot. You want to see a nice glow coming from the tip of that scalpel blade. Once you guys get a glowing, just set it off to the side and let it cool. Next. You want to take one of your guard dishes and have it ready. So what I like to do is take some rubbing alcohol on another paper towel and I like to wipe around the rim of my a guard dish. Next, you want to take your mushroom? You don't a mushroom and you want to cut it where the cap meets the stamp just like this. Once you detach the stem from the cap just like this, you could set the cap off to the side.
Next, two guys want to take your stem just like this. And as you guys can see, it already started bruising from where we detached it from the cap, which is a really good sign. Now, if you guys are looking at this directly on, you guys could see that tissue that's in the center of the stem. That's where you want to take a sample from. So you want to detach it from the cap and then you want to take a tissue sample from the center of the stem. This is the most sterile part of the entire mushroom and you're going to get a good tissue sample from there. It wasn't exposed to the elements. We just cut it open right now. So what you want to do is you want to take it freshly flame, sterilized scalpel blade. You want to start to cut out that tissue from the center of the stem. Just like this. Be very careful once you guys have a tissue sample. You're all set to put it to agar. Now, it doesn't need to be a perfect circle. Doesn't have to need to be a perfect line. You pretty much just want a nice piece of tissue sample. Now, it doesn't need to be a perfect circle or a perfect strip. You just want a nice, healthy piece of tissue. Once you guys have your tissue sample. Now you guys could put it to a. So I lift up just the corner of my lid and I police it right on the agar.
Once it's on a guard, you guys are all set. Now, you guys could put it off to the side. All right, guys. So that was one method. So pretty much we detach the stem from the cap and we take some tissue directly with stem met the cap. That's one method. Here's another method that I like to use if the stems of things. So this I like to use for Pinelli Sciences or any type of mushrooms that have much thinner stems, pretty much you want to do the same exact thing we did in the beginning. You want to sanitize your hands. You want to flame, sterilize a scalpel and get ready to take your tissue sample. Next, you want to take the donor mushroom and then what you want to do is want to take your freshly flame sterilized scalpel and you want to slice the stem directly down the middle. Once you guys have it sliced all the way down the middle. Now you want to open it up just like this, exposing all the tissue that's in the center of the stem. Next, you want to take a scrape or a cut from the stem? Once you guys have a nice sample. Now you guys can put it to Agar. Once you guys have it on agar, it's all set. You can put it off. The side edge, you guys could see this is the two samples we took. One is slightly longer than the other because we used two different methods of taking the tissue sample, but they both look great and they'll both do what they're supposed to do.
Like I said, guys, you want to be extremely sterile. You want to make sure you're working under a still air box or some type of filter like myself. Now, once you guys have the agar plates all set, you have tissue samples on it. You guys could label. I like to label them on the backside just so it doesn't interfere with my visual of what's going on on the plate. Now, after you guys take these samples in a few days to a week has passed. This is which you'll end up with. As you guys could see, the mycelium has started to grow on the agar. And it looks really nice, aggressive and healthy. It looks really, really good. And this is what you guys should expect. Now, you guys want to be extremely sterile when doing this. You know, it's very important to take every necessary step to be sterile, because if you guys just wing it. Yeah, you might get lucky. It might grow well, whatever. But if you guys are sterile, you guys will have a really high success rate, which is very important because I don't know anybody that has the time or money to be wasting. Not knowing if they have a really good chance of success. So the more sterile you guys are, the higher the chance of success.
Now, like I told you guys, you should be working inside of a still air box or underneath some type of filter like myself. You guys don't want to be doing this out in the open because the chances of contamination are really high. Now, I'd like to let my mycelium grow well, almost to the edges of the dish before I actually do my first transfer. And as you guys could see, there's still some sectors in the mycelium. It's not going to be a perfect monoculture. There's still gonna be sectorand but there's an A B far less sector. And then if you guys use the multi-sport syringe or a multi-sport print, as you guys could see, I labeled it on the back. I always label my garnishes dishes on the back when I'm doing any type of tissue sample or anything like that because I like to see the growth. I don't want anything obstruct. And so I labeled it Fiji. P.S. Cubans is the deep dish to clone. That's how I label all of my dishes or b dish one dish to dish three depending on how many samples I take from that specific donor mushroom. And like I said, I like to let it grow all the way up almost to the edges before I do my first transfer ban.
And there you go, guys. That's how I clone my mushrooms. So you guys could do this with any mushrooms that you guys want to do it with? You guys could do it with sclerosis. You guys could go to the supermarket, pick up some white mushrooms, some shit talky, some oysters, some lions' mean whatever you guys want to clone. You guys could do it the same exact way. Like I said, you could even do it with sclerosis. Just cut it in half and take the tissue sample from the center of the sclerosis to let it grow out. And then you guys move on and start doing your sectary and all that good stuff. And then you could freedom out and eventually you guys will get exactly what you're looking for, which is a nice, consistent flush of fruits that looked like the original donor mushroom. So it's a really cool thing you guys could try. It's a lot of fun. Actually, most of my agar plates are from cloned mushrooms. They're from donor mushrooms. And then eventually I'll be able to fruit out that a guard dish and I'm going to get a ton of mushrooms that look exactly like that original or have strong characteristics of that original. You know, if you was to take a spore print from one of them, really good mushrooms, so you got a really good mushroom spore, printed it. You made some syringes. It's gonna have a really good genetics pool, which means a lot of the genetics inside that spore print are gonna be good. But there's no guarantee that the two spores that germinate will create the same exact genetics that created that really good mushroom.
That's not to say you can't create a better one or a bunch of really good ones, but if you guys weren't really consistent flushers, you guys need to start getting into isolation and cloning. Most important step to have a nice consistent quality flushes. Like I said, you guys could do this with any supermarket farmer's market growing main mushroom, any type of mushroom that you guys could possibly dream of. You're gonna take a tissue sample the same exact way, put it to a agar, let it grow out, and then you start your cultivation and isolation processes from there. Eventually, if you guys follow every step, you're very diligent and you follow it all the way through. Eventually you will be rewarded with a beautiful flush of consistent mushrooms. You'll have them nice pen sets them really dense forest flushes that all you guys are looking for that you see in my Instagram post and all that stuff. That's what you guys will get if you start cloning. So definitely cloning is a must for. A mycologist, inspiring mycologist or just your, you know, weekend warrior. You know, someone that does a little bit girl on the weekend. You could do this, too. So definitely get into it. It's a lot of fun. It's well worth it if you want to bring things to the next level. This is the way to do it. Hopefully you guys enjoyed this video if you did do all that stuff I told you to do at the beginning. I love you guys. I'm really micro- do good. Be good. Live. Good. NAHMIAS Mr..
Next, two guys want to take your stem just like this. And as you guys can see, it already started bruising from where we detached it from the cap, which is a really good sign. Now, if you guys are looking at this directly on, you guys could see that tissue that's in the center of the stem. That's where you want to take a sample from. So you want to detach it from the cap and then you want to take a tissue sample from the center of the stem. This is the most sterile part of the entire mushroom and you're going to get a good tissue sample from there. It wasn't exposed to the elements. We just cut it open right now. So what you want to do is you want to take it freshly flame, sterilized scalpel blade. You want to start to cut out that tissue from the center of the stem. Just like this. Be very careful once you guys have a tissue sample. You're all set to put it to agar. Now, it doesn't need to be a perfect circle. Doesn't have to need to be a perfect line. You pretty much just want a nice piece of tissue sample. Now, it doesn't need to be a perfect circle or a perfect strip. You just want a nice, healthy piece of tissue. Once you guys have your tissue sample. Now you guys could put it to a. So I lift up just the corner of my lid and I police it right on the agar.
Once it's on a guard, you guys are all set. Now, you guys could put it off to the side. All right, guys. So that was one method. So pretty much we detach the stem from the cap and we take some tissue directly with stem met the cap. That's one method. Here's another method that I like to use if the stems of things. So this I like to use for Pinelli Sciences or any type of mushrooms that have much thinner stems, pretty much you want to do the same exact thing we did in the beginning. You want to sanitize your hands. You want to flame, sterilize a scalpel and get ready to take your tissue sample. Next, you want to take the donor mushroom and then what you want to do is want to take your freshly flame sterilized scalpel and you want to slice the stem directly down the middle. Once you guys have it sliced all the way down the middle. Now you want to open it up just like this, exposing all the tissue that's in the center of the stem. Next, you want to take a scrape or a cut from the stem? Once you guys have a nice sample. Now you guys can put it to Agar. Once you guys have it on agar, it's all set. You can put it off. The side edge, you guys could see this is the two samples we took. One is slightly longer than the other because we used two different methods of taking the tissue sample, but they both look great and they'll both do what they're supposed to do.
Like I said, guys, you want to be extremely sterile. You want to make sure you're working under a still air box or some type of filter like myself. Now, once you guys have the agar plates all set, you have tissue samples on it. You guys could label. I like to label them on the backside just so it doesn't interfere with my visual of what's going on on the plate. Now, after you guys take these samples in a few days to a week has passed. This is which you'll end up with. As you guys could see, the mycelium has started to grow on the agar. And it looks really nice, aggressive and healthy. It looks really, really good. And this is what you guys should expect. Now, you guys want to be extremely sterile when doing this. You know, it's very important to take every necessary step to be sterile, because if you guys just wing it. Yeah, you might get lucky. It might grow well, whatever. But if you guys are sterile, you guys will have a really high success rate, which is very important because I don't know anybody that has the time or money to be wasting. Not knowing if they have a really good chance of success. So the more sterile you guys are, the higher the chance of success.
Now, like I told you guys, you should be working inside of a still air box or underneath some type of filter like myself. You guys don't want to be doing this out in the open because the chances of contamination are really high. Now, I'd like to let my mycelium grow well, almost to the edges of the dish before I actually do my first transfer. And as you guys could see, there's still some sectors in the mycelium. It's not going to be a perfect monoculture. There's still gonna be sectorand but there's an A B far less sector. And then if you guys use the multi-sport syringe or a multi-sport print, as you guys could see, I labeled it on the back. I always label my garnishes dishes on the back when I'm doing any type of tissue sample or anything like that because I like to see the growth. I don't want anything obstruct. And so I labeled it Fiji. P.S. Cubans is the deep dish to clone. That's how I label all of my dishes or b dish one dish to dish three depending on how many samples I take from that specific donor mushroom. And like I said, I like to let it grow all the way up almost to the edges before I do my first transfer ban.
And there you go, guys. That's how I clone my mushrooms. So you guys could do this with any mushrooms that you guys want to do it with? You guys could do it with sclerosis. You guys could go to the supermarket, pick up some white mushrooms, some shit talky, some oysters, some lions' mean whatever you guys want to clone. You guys could do it the same exact way. Like I said, you could even do it with sclerosis. Just cut it in half and take the tissue sample from the center of the sclerosis to let it grow out. And then you guys move on and start doing your sectary and all that good stuff. And then you could freedom out and eventually you guys will get exactly what you're looking for, which is a nice, consistent flush of fruits that looked like the original donor mushroom. So it's a really cool thing you guys could try. It's a lot of fun. Actually, most of my agar plates are from cloned mushrooms. They're from donor mushrooms. And then eventually I'll be able to fruit out that a guard dish and I'm going to get a ton of mushrooms that look exactly like that original or have strong characteristics of that original. You know, if you was to take a spore print from one of them, really good mushrooms, so you got a really good mushroom spore, printed it. You made some syringes. It's gonna have a really good genetics pool, which means a lot of the genetics inside that spore print are gonna be good. But there's no guarantee that the two spores that germinate will create the same exact genetics that created that really good mushroom.
That's not to say you can't create a better one or a bunch of really good ones, but if you guys weren't really consistent flushers, you guys need to start getting into isolation and cloning. Most important step to have a nice consistent quality flushes. Like I said, you guys could do this with any supermarket farmer's market growing main mushroom, any type of mushroom that you guys could possibly dream of. You're gonna take a tissue sample the same exact way, put it to a agar, let it grow out, and then you start your cultivation and isolation processes from there. Eventually, if you guys follow every step, you're very diligent and you follow it all the way through. Eventually you will be rewarded with a beautiful flush of consistent mushrooms. You'll have them nice pen sets them really dense forest flushes that all you guys are looking for that you see in my Instagram post and all that stuff. That's what you guys will get if you start cloning. So definitely cloning is a must for. A mycologist, inspiring mycologist or just your, you know, weekend warrior. You know, someone that does a little bit girl on the weekend. You could do this, too. So definitely get into it. It's a lot of fun. It's well worth it if you want to bring things to the next level. This is the way to do it. Hopefully you guys enjoyed this video if you did do all that stuff I told you to do at the beginning. I love you guys. I'm really micro- do good. Be good. Live. Good. NAHMIAS Mr..
